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Iba1 Rabbit Monoclonal Antibody

Cat Number: MABN21499
Conjugate: Unconjugated
Size: 100μL
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG,Kappa
Reactivity: Human,Mouse,Rat
Applications: WB 1:1000-1:5000,IHC 1:2000-1:4000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200
Molecular: Calculated MW:17kD;Observed MW:17kD
Purification: Affinity purification
Synonyms: AIF 1;AIF-1;Aif1;AIF1 protein;AIF1_HUMAN;;BART 1;G1;G11;IBA 1;IBA1;interferon gamma responsive transcript;Interferon responsive transcript 1;interferon responsive transcript factor 1, RT 1;IRT1;Protein g1;
Background:

Cell localization:Cytoplasm.This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016],

Form: liquid
Buffer: PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Western blot analysis of lysates from Mouse spleen cells, using Iba1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Western blot analysis of lysates from Mouse spleen cells, using Iba1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded Human liver tissue. 1, Iba1 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human liver tissue. 1, Iba1 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue. 1, Iba1 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue. 1, Iba1 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).