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IBA1 Rabbit Polyclonal Antibody

Cat Number: AB-83747
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from part region of human protein
Reactivity: Human,Mouse,Rat
Applications:

Western Blot: 1/500 – 1/2000
Immunofluorescence: 1:100-300
ELISA: 1/5000
Molecular Weight: 16kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background:

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and
T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-83747-1

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

AB-83747-2

Immunofluorescence analysis of A549.
1,primary Antibody was diluted at 1:200(4°C overnight).
2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.