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IFN gamma

Cat Number: AB-83768
Conjugate: Unconjugated
Size: 100 ug
Clone: poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200

Molecular: 17-23kDa
Purification: Aff.Pur.
Synonyms: IFNG;IFG;IFI
Background:

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83768-1.jpg

Western blot analysis of extracts of
various cell lines, using IFNG antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 60s.

AB-83768-2.jpg

Immunohistochemistry of paraffin embedded
human lung using IFNG
antibody at dilution of
1:100 (40x lens).