IFN gamma
| Cat Number: | AB-83768 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 17-23kDa |
| Purification: | Aff.Pur. |
| Synonyms: | IFNG;IFG;IFI |
| Background: | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using IFNG antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 60s.
Immunohistochemistry of paraffin embedded
human lung using IFNG
antibody at dilution of
1:100 (40x lens).