Insulin Rabbit Polyclonal Antibody
| Cat Number: | AB-84377 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:2000 Immunohistochemistry: 1:50 – 1:200 Immunofluorescence: 1:50 – 1:200 |
| Molecular Weight: | 12kDa |
| Purification: | Affinity purification |
| Synonyms: | IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin |
| Background: | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5′ region and with the IGF2 gene at the 3′ region. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteInsulin: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin embedded rat pancreatic islet using Insulin antibody at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin embedded mouse pancreatic islet using Insulin antibody at dilution of 1:50 (40x lens).
Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb at dilution of 1:100.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse pancreatic islet using Insulin Rabbit pAb at dilution of 1:100.
Blue: DAPI for nuclear staining.
References:
References for Insulin Rabbit pAb
Product:Insulin Rabbit pAb
Journal:Cell death & disease
Application:IHC
IF:5.964
Species:Mus musculus
PMID:28837146
Title:Adropin deficiency worsens HFD-induced metabolic defects
References for Insulin Rabbit pAb
Product:Insulin Rabbit pAb
Journal:Heliyon
Application:IHC
IF:
Species:Homo sapiens
PMID:31989054
Title: The serum concentration of βCGRP is novel marker for type 1 diabetes