Integrin a1 (Cytoplasmatic)
|Hu, Ms, Rt, Ch, Ha
Western Blot: 1:1000
Integrins are a family of membrane glycoproteins that include receptors for fibronectin, laminin, collagens vitronectin and fibrinogen. These receptors mediate adhesive interactions of the cells during development and tissue repair. The integrins consist of heterodimeric complexes of and subunits. The family includes 17 different and 8 different that can associate to form distinct receptor complexes. These receptors are expressed in different cell type and each integrin complexes perform different function. Most of the integrins are expressed also on leukocytes where they are known as VLA antigens. The 1 subunit associates with 1 and it forms a receptors for collagens and laminin. The antibody to 1 is useful for a variety of studies such as analysis of the distribution and expression by western blotting, immunofluorescence and immunoprecipitation. This antibody is usefull for analysis of 1 in all animal species. Antigen: NH2-KIGFKRPLKKKMEK-COOH synthetic peptide derived from the COOH terminal region of the human protein (cytoplasmic domain) coupled to KLH. This region of the molecule is localized intracellularly.
|The antiserum contains 0.05% sodium azide as preservative. At this concentration azide will be toxic to most cellular systems. If necessary it can be removed through dialysis against an appropriate buffer prior to use.
|The antiserum can be stored frozen at -20°C in small aliquots.