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CD25 Rabbit Polyclonal Antibody

Cat Number: AB-81365
Conjugate: Unconjugated
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD25.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry (paraffin-embedded tissues): 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Flow Cytometry: C 1:50 – 1:200
Molecular Weight: 60KDa
Purification: Aff. Pur.
Synonyms: p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Background:

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA
and IL2RB gene polymorphisms have been associated with lung cancer risk.
Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-81365-1

Western blot analysis of extracts of various cell lines, using CD25 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

AB-81365-2

Immunohistochemistry of paraffinembedded human tonsil using CD25 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
protocol.

AB-81365-3

Immunohistochemistry of paraffinembedded rat spleen using CD25 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-81365-4

Immunofluorescence analysis of rat spleen cells using CD25 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for
nuclear staining.

AB-81365-5

Flow cytometry: 293F Transfection cells(+) and 293F cells(-) were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD25 Rabbit pAb 2.5ug/ml orange line), followed by Goat anti-Rabbit pAb Alexa Fluor 647 (1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).

References:

References for CD25 Rabbit pAb
Product:CD25 Rabbit pAb
Journal:Journal of nanobiotechnology
Application:IHC
IF:10.43
Species:Mus musculus
PMID:35248056
Title:Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress-activated NF-κB pathway