JAK2 Rabbit Polyclonal Antibody
| Cat Number: | AB-81994 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human JAK2. AA range:981-1030 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 130kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2 |
| Background: | This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of HT29 cells using JAK2 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from HT-29 cells, using JAK2 Antibody.
The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue.
1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-lung tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B