JNK Rabbit Polyclonal Antibody

Cat Number: AB-82391
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3.
Reactivity: Hu, Ms, Rt

Western Blot:                        1:500 – 1:2000
Immunohistochemistry:     1:50 – 1:200

Immunofluorescence:         1:50 – 1:200        Immunocytochemistry:      1:50 – 1:200
Immunoprecipitation:        1:500 – 1:1000

Molecular Weight: 46kDa/54kDa
Purification: Aff. Pur.

JNK belongs to MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1,JNK2,and JNK3.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.


Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 antibody.                                                                       Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody at a dilution of 1:1000.


Immunohistochemistry of paraffinembedded human lung cancer using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC
staining protocol.


Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).                                                                    Blue: DAPI for nuclear staining.


References for JNK1/2/3 Rabbit pAb
Product:JNK1/2/3 Rabbit pAb
Title:  Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity


References for JNK1/2/3 Rabbit pAb
Product:JNK1/2/3 Rabbit pAb
Species:Bos taurus
Title:Transcriptome Analysis Reveals That NEFA and β-Hydroxybutyrate Induce Oxidative Stress and Inflammatory Response in Bovine Mammary Epithelial Cells – PMC