Ki-67
| Cat Number: | AB-84220 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human Ki67 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 358kDa |
| Purification: | Aff. Pur. |
| Synonyms: | MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of prolif eration Ki-67;Ki67 |
| Background: | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
NIH/3T3 cells, using Ki67 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunofluorescence analysis of C6
cells using Ki67 antibody at
dilution of 1:100. Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of U2OS cells using Ki67 antibody at
dilution of 1:100. Blue: DAPI for
nuclear staining.