KI-67
| Cat Number: | MAB-94467 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | Mib1 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Nuclei of the Hodgkin lymphoma cell line L428 |
| Reactivity: | Hu |
| Applications: | Immunohistochemistry , Western Blot , Flow Cytometry (QC tested), Immunocytochemistry |
| Purification: | Aff. Pur. |
| Synonyms: | KIA, MIB, Marker of proliferation Ki67, MKI67 |
| Background: | The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells. Ki-67 antigen is a nuclear Protein can recognize both isoforms with moelcular weight: 345 kDa and 395 kDa. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Storage: | Store at 2-8°C. Do not freeze. For longer term store at: -20°C |
Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue)
References
Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22.
PubMed
Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21.
PubMed
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5.
PubMed
Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206.
PubMed
Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20.