[KO Validated] NF-kB p65/RelA Rabbit Monoclonal Antibody
| Cat Number: | MAB-94597 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC51086 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat,Monkey |
| Applications: | WB 1:5000 - 1:20000 IHC-P 1:2000 - 1:8000 IF/ICC 1:600 - 1:2400 ChIP 5μg antibody for 10μg-15μg of Chromatin ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular: | 65kDa |
| Purification: | Affinity purification |
| Synonyms: | p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA |
| Background: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| Form: | liquid |
| Buffer: | PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
![Western blot analysis of lysates from wild type (WT) and NF-kB p65/RelA knockout (KO) HeLa cells using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.](https://immunologicalsciences.com/wp-content/uploads/2025/10/product_294_img_1.jpg)
Western blot analysis of lysates from wild type (WT) and NF-kB p65/RelA knockout (KO) HeLa cells using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
![Western blot analysis of lysates from PC-12 cells using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.](https://immunologicalsciences.com/wp-content/uploads/2025/10/product_294_img_2.jpg)
Western blot analysis of lysates from PC-12 cells using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
![Immunohistochemistry analysis of paraffin-embedded HT-1080 cell lines(untreated and treated with TNF-α) using [KO Validated] NF-kB p65/RelA Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](https://immunologicalsciences.com/wp-content/uploads/2025/10/product_294_img_3-1024x510.jpg)
Immunohistochemistry analysis of paraffin-embedded HT-1080 cell lines(untreated and treated with TNF-α) using [KO Validated] NF-kB p65/RelA Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.