KU70
| Cat Number: | AB-82007 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human KU70. |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 -1:2000 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pur. |
| Synonyms: | XRCC6; CTC75; CTCBF; G22P1; KU70; ML8; TLAA; X -ray repair cross complementing 6 |
| Background: | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a singles-tranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of various cell lines, using KU70 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody ) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KU70 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.