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KU70

Cat Number: AB-82007
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human KU70.
Reactivity: Hu,Ms,Rt
Applications:

Western Blot: 1:500 -1:2000
Immunohistochemistry: 1:50 -1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 70kDa
Purification: Aff. Pur.
Synonyms: XRCC6; CTC75; CTCBF; G22P1; KU70; ML8; TLAA; X -ray repair cross complementing 6
Background:

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a singles-tranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-812007-1.jpg

Western blot analysis of extracts of various cell lines, using KU70 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

AB-812007-2.jpg

Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody ) at dilution of 1:100 (40x lens).

AB-812007-3.jpg

Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody at dilution of 1:100 (40x lens).

AB-812007-4.jpg

Immunofluorescence analysis of HeLa cells using KU70 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.