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KU80

Cat Number: AB-11718
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human KU86
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 -1:2000
Immunohistochemistry: 1:100 -1:200
Immunofluorescence: 1:20 -1:100
Immunoprecipitation: 1:50 – 1:200

Molecular: 86kDa
Purification: Purified
Synonyms: KU86; KARP-1; KARP1; KU80; KUB2; Ku86; NFIV;X-ray repair cross complementing 5
Background:

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein KU86. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA doublestrand break by non-homologous end joining and the completion of V(D)Jrecombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-11718-1.jpg

Western blot analysis of extracts of various cell lines, using KU86 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-11718-2.jpg

Immunohistochemistry of paraffin-embedded human breast cancer using KU86 Antibody at dilution of 1:100 (40x lens).

AB-11718-3.jpg

Immunohistochemistry of paraffin-embedded human gastric cancer using KU86 Antibody at dilution of 1:100 (40x lens).