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LAMP2

Cat Number: AB-84316
Conjugate: Unconjugated
Size: 100 μg
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-300 of human LAMP2.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200

Molecular: 110-130kDa
Purification: Aff. Pur.
Synonyms: LAMP2;CD107b;LAMP-2;LAMPB;LGP-96;LGP110
Background:

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84316-1.jpg

Western blot analysis of extracts of various cell lines, using LAMP2 antibody ) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.

AB-84316-2.jpg

Western blot analysis of extracts of various cell lines, using LAMP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84316-3.jpg

Immunohistochemistry of paraffin embedded human gastric cancer using
LAMP2 Antibody at dilution of
1:100 (40x lens).