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LAMP3 /CD63

Cat Number: AB-83481
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63
Reactivity: Hu
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200

Molecular: 30-60kDa
Purification: Aff. Pur.
Synonyms: CD63;LAMP-3;ME491;MLA1;OMA81H;TSPAN30;CD63 antigen;CD63
Background:

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83481-1.jpg

Western blot analysis of extracts of various cell line, using CD63
at 1:1,000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit.
Exposure time: 15s

AB-83481-2.jpg

Western blot analysis of extracts of various cell lines, using CD63 antibody (at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.

AB-83481-3.jpg

Immunohistochemistry of paraffinembedded
rat brain using CD63 Rabbit pAb at dilution of 1:100 (40x lens).

AB-83481-4.jpg

Review for CD63 Rabbit pAb
Experiment Type: Western blot(WB)

AB-83481-5.jpg

Review for CD63 Rabbit pAb

References