MAP-2ab
| Cat Number: | AB-10417 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 50 ul |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Ch |
| Isotype: | IgY |
| Immunogen: | Full length native protein (purified) corresponding to Cow MAP2. Positive control: ICC: cultured rat cortical neurons.Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. |
| Reactivity: | Hu, Rt, Ms, Bv, Mamm |
| Applications: | Western Blot: 1:10000-1:100.000 |
| Molecular Weight: | ~280kDa |
| Purification: | Serum |
| Form: | Liquid |
| Buffer: | Preservative: 0.02% Sodium azide |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
Western blot – Anti-MAP2 antibody
All lanes : Anti-MAP2 antibody at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody PFA-fixed, paraffin embedded sections of sheep cerebellum were stained for MAP2 with AB-10147 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) was used as secondary antibody at 1/200 dilution. Immunocytochemistry/ Immunofluorescence – Anti-MAP2 antibody Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using AB-10147 at 1/2000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
Boiling in citrate-buffer was used as antigen retrieval method. AB-10147 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
Boiling in citrate-buffer was used as antigen retrieval method. AB-10147 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.