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MAP-2ab

Cat Number: AB-10417
Conjugate: Unconjugated
Size: 50 ul
Clone: POLY
Concentration: 1mg/ml
Host: Ch
Isotype: IgY
Immunogen: Full length native protein (purified) corresponding to Cow MAP2. Positive control: ICC: cultured rat cortical neurons.Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
Reactivity: Hu, Rt, Ms, Bv, Mamm
Applications:

Western Blot: 1:10000-1:100.000
This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.
Immunocytochemistry: 1:5000-10.000
Immunofluorescence: 1:5000-10.000
Immunohistochemistry (frozen tissues)1:5000-10.000
Immunohistochemistry (paraffin-embedded tissues:To be optimized by end user.
ELISA: 1:5000

Molecular: ~280kDa
Purification: Serum
Form: Liquid
Buffer: Preservative: 0.02% Sodium azide
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
AB-10417-MAP2A-B-1.jpg

Western blot – Anti-MAP2 antibody
All lanes : Anti-MAP2 antibody at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate

AB-10417-MAP2A-B-2.jpg

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody PFA-fixed, paraffin embedded sections of sheep cerebellum were stained for MAP2 with AB-10147 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) was used as secondary antibody at 1/200 dilution. Immunocytochemistry/ Immunofluorescence – Anti-MAP2 antibody Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using AB-10147 at 1/2000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
Boiling in citrate-buffer was used as antigen retrieval method. AB-10147 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.

AB-10417-MAP2A-B-3.jpg

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-MAP2 antibody staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
Boiling in citrate-buffer was used as antigen retrieval method. AB-10147 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.