|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 360-719 of human MCM7|
Western Blot: 1:500 – 1:2000
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles|
Western blot analysis of extracts of
various cell lines, using MCM7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunofluorescence analysis of HeLa
cells using MCM7 antibody.
Blue: DAPI for nuclear staining.