MCP-1
| Cat Number: | AB-80184 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human MCP-1 |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 11kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CCL2;GDCF-2;HC11;HSMCR30;MCAF;MCP-1;MCP1;SCYA2 ;SMC-CF |
| Background: | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of
various cell lines, using CCL2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk inTBST.
Detection: ECL Wes Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffin embedded human colon using CCL2;
antibody at dilution of 1:100
(40x lens).
Immunofluorescence analysis of U-2 OS cells using CCL2; antibody at
dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CCL2;antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Review for CCL2;Rabbit
Experiment Typemmunofluorescence (IF)
Samplerat brain cells
Description Immunofluorescence analysis of rat brain cells using antibody at 1:50 dilution. The positive signal is Golgi staining.
References
Product: CCL2;Rabbit pAb
Journal:tumor biology
Application:WB IF:3.649
Species:Homo sapiens PMID: 28347237
Title: Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment
Product: CCL2;Rabbit pAb
Journal:Vascular Pharmacology
Application:WB IF:.715
Species:Mus musculus
PMID:28069546
Title: G004, a synthetic sulfonylurea compound, exerts anti-atherosclerosis effects by targeting SIRT1 in ApoE(-/-) mice
References for MCP-1 Rabbit pAb
Product: CCL2; Rabbit pAb
Journal:Bioscience reports
Application:WB, IHC
IF:2.89 Species:
Rattus norvegicus PMID:
30760630 Title:Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages.