MKL1
| Cat Number: | AB-84312 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids1-280 of human MKL1. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 145kDa |
| Purification: | Aff. Pur. |
| Synonyms: | MKL1; BSAC; MAL; MRTF-A |
| Background: | The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator ofsmooth muscle cell differentiation. The encoded protein is predominantly nuclear and may helptransduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocationevent that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocationhas been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using MKL1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using MKL1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cell susing MKL1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.