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MNF1 Polyclonal Antibody

Cat Number: AB-84439
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids14-126 of human MNF1.
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence / Immunocytochemistry 1:50 – 1:200
Molecular Weight: 15KDa
Purification: Affinity purification
Synonyms: LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
Background:

The product of this gene is a subunit of neuronal microtubule-associated MAP1A andMAP1B proteins, which are involved in microtubule assembly and important for
neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Form: Liquid
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
84439

Western blot analysis of extracts from normal (control) and UQCC2 knockout (KO) 293T cells, using
MNF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.