mTOR
Cat Number: | MAB-94142 |
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Conjugate: | Unconjugated |
Size: | 100 ul |
Clone: | D9C2 |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Recombinant protein of human MTOR |
Reactivity: | Hu, Ms, Rt |
Applications: | Western Blot: 1:500-1:1,000 |
Molecular Weight: | 288kDa |
Purification: | Aff, Pur. |
Background: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinaserelated kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
HeLa cells, using mTOR antibody dilution: 1:500.
Secondary antibody:
HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.