|Immunogen:||Recombinant protein of human MTOR|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500-1:1,000
The protein encoded by this gene belongs to a family of phosphatidylinositol kinaserelated kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of
HeLa cells, using mTOR antibody dilution: 1:500.
HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.