mTOR
| Cat Number: | AB-83580 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR |
| Reactivity: | Hu,Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | Calculated MW: 288kDa Observed MW: 290kDa |
| Purification: | Aff, Pur. |
| Synonyms: | MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin |
| Background: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
| Form: | Liquid |
| Buffer: | Liquid in PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Femto Plus
Exposure time: 15s
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody Western blot was performed from the immunoprecipitate using mTOR antibody at a dil. of 1:100
Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.