|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR|
Western Blot: 1:500 – 1:1000
|Molecular:||Calculated MW: 288kDa Observed MW: 290kDa|
|Synonyms:||MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin|
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
|Buffer:||Liquid in PBS with 0.02% sodium azide, 50% glycerol, pH7.3|
|Storage:||At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles.|
Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Femto Plus
Exposure time: 15s
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody Western blot was performed from the immunoprecipitate using mTOR antibody at a dil. of 1:100
Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.