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mTOR

Cat Number: AB-83580
Conjugate: Unconjugated
Size: 100 ul
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR
Reactivity: Hu,Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunoprecipitation: 1:50

Molecular: Calculated MW: 288kDa Observed MW: 290kDa
Purification: Aff, Pur.
Synonyms: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin
Background:

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Form: Liquid
Buffer: Liquid in PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage: At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles.
AB-83580-1.jpg

Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Femto Plus
Exposure time: 15s

AB-83580-2.jpg

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody Western blot was performed from the immunoprecipitate using mTOR antibody at a dil. of 1:100

AB-83580-3.jpg

Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

AB-83580-4.jpg

Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.