Myelin Associated Glycoprotein (MAG)

Cat Number: AB-81354
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MAG(114-132aa KYYFRGDLGGYNQYTFSEH), identical to therelated rat and mouse sequences.
Reactivity: Hu, Ms, Rat

Western blot: 1:1000-1:12000
Immunohistochemistry (Paraffin-embedded Section): 1:100-1:200

Purification: Aff. Pur.

MAG(Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membraneglycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgRexpressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney(HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

Form: Liquid
Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Storage: At -20°C for one year. Avoid repeated freezing and thawing.

Anti-MAG antibody, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate


IHC(P): Human Mammary Cancer Tissue


Anti-MAG antibody, IHC(P)
IHC(P): Rat Brain Tissue