Western Blot: 1:500-1:2000
|Purification:||Aff. Pur. ≥95% as determined by SDS-PAGE|
Transcription regulator involved in inner cell mass and embryonic stem(ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5′-TAAT[GT][GT]-3′ or 5′-[CG][GA][CG]C[GC]ATTAN[GC]-3′. Able to autorepress its expression in differentiating(ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
|Buffer:||PBS with 0.02% sodium azide and 50% glycerol pH 7.3|
|Storage:||T -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)|
rat brain tissue were subjected to SDS PAGE followed by western blot with NANOG Antibody at dilution of 1:2000
Immunofluorescent analysis of NCCIT cells, using Nanog