NEFM Mouse Monoclonal Antibody
| Cat Number: | MABN81800 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ul |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | Mouse IgG1 |
| Immunogen: | Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli. |
| Reactivity: | Human |
| Applications: | ICC 1:50-1:200,ELISA 1:5000-1:20000,FC 1:200-1:400 |
| Molecular: | 5kDa |
| Purification: | Affinity purification |
| Synonyms: | NFM; NEF3; NF-M |
| Background: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Form: | liquid |
| Buffer: | Purified antibody in PBS with 0.05% sodium azide |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).