NELFE Rabbit Monoclonal Antibody
| Cat Number: | MABN02330 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | Monoclonal |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human NELFe |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,IP 1:20-1:50 |
| Molecular: | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Purification: | Affinity Purification |
| Synonyms: | RD; RDP; RDBP; D6S45; NELF-E |
| Background: | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. |
| Form: | Liquid |
| Buffer: | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles. |