|Immunogen:||synthetic peptide corresponding to residues near the carboxy terminus of human NeuN protein.|
|Reactivity:||Hu, Ms, Rt|
WB: 1:1000, IF: 1:500, ICC, IHC(F): To be determined by end user
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).
|Storage:||4°C for short term and -20°C for longer term|
Western blot analysis of extracts from mouse brain, C2C12
cells, and rat brain using NeuN (D3S3I) Rabbit mAb (upper) or
b-Actin (D6A8) Rabbit mAb (lower).
Confocal immunofluorescent analysis of normal mouse cerebellum using NeuN (D3S3I) Rabbit mAb (green),
and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).