Neurofilament heavy polypeptide Rabbit Monoclonal Antibody
| Cat Number: | MABN21532 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ul |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Immunogen: | A synthetic peptide of human Neurofilament heavy polypeptide |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:1000-1:5000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200 |
| Molecular: | Calculated MW:110kD;Observed MW:180-200kD |
| Purification: | Affinity purification |
| Synonyms: | NEFH;KIAA0845;NFH;Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein |
| Background: | Cell localization:Cytoplasm.Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008], |
| Form: | Liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of lysates from Mouse brain cells, using Neurofilament heavy polypeptide Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, Neurofilament heavy polypeptide Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue. 1, Neurofilament heavy polypeptide Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).