NFKB p65
| Cat Number: | AB-82033 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:1000 |
| Molecular Weight: | 65 kDa |
| Purification: | Aff. Pur. |
| Synonyms: | RELA; NFKB3; p65; transcription factor p65 |
| Background: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using NF-kB p65
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using NF-kB p65 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NF-kB p65 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NF-kB p65 antibody.
Application: Western blot (WB)
Sample: RAW264.7
Loading Amount : 15 ug
Dilution: 1:500
Incubation time: over night
Temperature 4 ℃
Exposure: 15 seconds
Observed Band: 65 kDa
Remarks Western blot analysis of extracts of mouse atherosclerosis arterial tissue, using RELA antibody at 1:500 dilution.The band is specific and clear.
References
References:
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Journal of Immunology
Application:WB
IF:4.849
Species:Human
PMID:26202983
Title:MicroRNA-15b Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF125
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal: Journal Of Proteome Research
Application:WB
IF:4.267
Species:Human
PMID:26485063
Title:Quantitative Label-Free Phosphoproteomics Reveals Differentially Regulated Protein Phosphorylation Involved in West Nile Virus-Induced Host Inflammatory Response
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Journal Of Proteomics
Application:WB IF:
3.913 Species:
African green monkey
PMID:
26361011 Title:iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Scientific Reports
Application:WB
IF:4.258
Species:Human
PMID:26931521
Title:Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Cell Death And Differentiation
Application:ChIP
IF:8.329
Species:Human
PMID:28475173
Title:MicroRNA-30a attenuates mutant KRAS-driven colorectal tumorigenesis via direct suppression of ME1
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Molecular immunology
Application:WB
IF:3.235
Species:Human
PMID:28073078
Title:TRIM52: A nuclear TRIM protein that positively regulates the nuclear factor-kappa B signaling pathway
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:FASEB Journal
Application:WB
IF:5.498
Species:Rat
PMID:29401609
Title:MicroRNA-149-5p regulates blood–brain barrier permeability after transient middle cerebral artery occlusion in rats by targeting S1PR2 of pericytes
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Cytokine
Application:WB
IF:3.488
Species:Rat
PMID:29549724
Title:Berberine inhibits IL-21/IL-21R mediated inflammatory proliferation of fibroblast-like synoviocytes through the attenuation of PI3K/Akt signaling pathway and …
References for NF-kB p65 Polyclonal Antibody
Product:NF-kB p65 Polyclonal Antibody
Journal:Neuroscience letters
Application:IF
IF:2.15
Species:Rat
PMID:30075286
Title:Sulfasalazine attenuates chronic constriction injury-induced neuroinflammation and mechanical hypersensitivity in rats