NG2 /CSPG4 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94805 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC64580 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1583-2224 of human NG2. |
| Reactivity: | Human,Mouse |
| Applications: | Western Blot 1:500 – 1:1000 |
| Molecular Weight: | 251 kDa |
| Purification: | Affinity purification |
| Synonyms: | NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; NG2/CSPG4 |
| Background: | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. |
| Form: | Liquid |
| Buffer: | Recombinant fusion protein containing a sequence corresponding to amino acids 1583-2224 of human NG2 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western Blot image using NG2 on human samples.
Western Blot image using NG2 on mouse samples.
Immunohistochemistry analysis of NG2/CSPG4 in paraffin-embedded human colon carcinoma tissue using NG2/CSPG4 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of NG2/CSPG4 in paraffin-embedded human spleen tissue using NG2/CSPG4 Rabbit mAb
at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer
(pH 6.0) prior to IHC staining.
Confocal imaging of SK-MEL-28 cells using NG2/CSPG4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and SK-MEL-28 cells (right) were surface-stained with NG2/CSPG4 Rabbit mAb (2 μg/mL,orange line) or AF647 Rabbit IgG isotype control (5 μl/Test,blue line), followed by AF647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 SK-MEL-28 cells were surface-stained with AF647 Rabbit IgG isotype control (5 μl/Test,left) or NG2/CSPG4 Rabbit mAb (2 μg/mL,right).