NOS2(I/NOS) Rabbit Polyclonal Antibody
| Cat Number: | AB-80658 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 131kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2 |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from NIH/3T3 cells, using iNOS Antibody.
The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549.
1,primary Antibody(red) was diluted at 1:200(4°C overnight).
2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue.
1,NOS2 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.