NOS2(I/NOS) Rabbit Polyclonal Antibody

Cat Number: AB-80658
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
Reactivity: Hu, Ms, Rt

Western Blot: 1:500-2000
Immunofluorescence: 1:50-200
Immunohistochemistry: 1:50-300
ELISA: 1:10000-20000

Molecular Weight: 131kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric
oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.

Western blot analysis of lysates from NIH/3T3 cells, using iNOS Antibody.
The lane on the right is blocked with the synthesized peptide.


Immunofluorescence analysis of A549.
1,primary Antibody(red) was diluted at 1:200(4°C overnight).
2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.


Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue.
1,NOS2 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.