NOX2
| Cat Number: | AB-83889 |
|---|---|
| Size: | 100 ug |
| Clone: | poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human Nox2 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1 :500 – 1:1000 |
| Molecular Weight: | 65kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CYBB; AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX; cytochrome b-245 beta chain |
| Background: | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell’s respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | At 4°C for short term. For longer terms at -20°. Avoid freeze and thaw cycles |
Western blot analysis of extracts of various cell lines, using
Nox2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit Exposure time: 60s.
Immunohistochemistry of paraffin-embedded on Rat brain using NOX2 antibody with a dil: 1:100
Immunofluorescence analysis of A431 cells using Nox2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.