NOX4 Rabbit Polyclonal Antibody
| Cat Number: | AB-84210 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human NADPH oxidase 4 |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:100-1:200,FC 1:20-1:50 |
| Molecular: | 67kDa |
| Purification: | Affinity purification |
| Synonyms: | NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX |
| Background: | The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. |
| Form: | Liquid |
| Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Western blot analysis of NOX4 expression in JAR cell lysate.
Please find below information for reference.
Working dilution of the primary antibody 1: 1000
Working dilution of the Secondary antibody: 1: 8000
Samples of the experiments: Sample loading volume 25ul
Chemiluminescent substrate: ECL West Pico Plus
Notes for Western Blot:
Add protease inhibitors during sample processing and lyse samples with dedicated membrane protein lysis buffer. Do not boil the samples at high temperature;
All subsequent procedures following standard WB protocols would be fine.