NRF2 Rabbit Polyclonal Antibody
| Cat Number: | AB-84017 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605. |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 75-100kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Synonyms: | NFE2L2; NRF2; Nuclear factor erythroid 2-relatedfactor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2 |
| Background: | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from
1)Hela cell ,
2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUC
AAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour.
(Red) GAPDH Monoclonal Antibody (5B7)
antibody was diluted at 1:5000 as loading control, 4° over night, AF680 secondary antibody was diluted at 1:10000, 37° 1hour.
Anti-Inflammatory and Antioxidant Effects of Kelong-Capsule on Testosterone-Induced Benign Prostatic
Hyperplasia in Rats.” Evidence-Based Complementary and Alternative Medicine 2018.
“Glutamine ameliorates intestinal ischemiareperfusion Injury in rats by activating the Nrf2/Are signaling pathway.” International journal of clinical and experimental pathology 8.7 (2015): 7896
Immunofluorescence analysis of HUVEC cells, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the
right is blocked with the synthesized peptide.