Occludin
| Cat Number: | AB-83608 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human OCLN |
| Reactivity: | Hu, Ms ,Rt |
| Applications: | WB 1:1000 – 1:2000 |
| Molecular Weight: | 59kDa |
| Purification: | Aff. Pur. |
| Background: | This gene encodes an integral membrane protein that is required for ytokineinduced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Occludin
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.
Immunohistochemistry of paraffinn embedded human colon using
Occludin Rabbit pAb at
dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Occludin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Reviews for Occludin Rabbit Polyclonal Antibody (AB-83608)
Review for Occludin Rabbit pAb (AB-83608)