Top

Occludin

Cat Number: AB-83608
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human OCLN
Reactivity: Hu, Ms ,Rt
Applications:

WB 1:1000 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Molecular: 59kDa
Purification: Aff. Pur.
Background:

This gene encodes an integral membrane protein that is required for ytokineinduced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83608-1.jpg

Western blot analysis of extracts of various cell lines, using Occludin
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.

AB-83608-2.jpg

Immunohistochemistry of paraffinn embedded human colon using
Occludin Rabbit pAb at
dilution of 1:100 (40x lens).

AB-83608-3.jpg

Immunofluorescence analysis of HeLa cells using Occludin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

AB-83608-4.jpg

Reviews for Occludin Rabbit Polyclonal Antibody (AB-83608)

AB-83608-5.jpg

Review for Occludin Rabbit pAb (AB-83608)