|Immunogen:||Recombinant protein of human OCLN|
|Reactivity:||Hu, Ms ,Rt|
WB 1:1000 – 1:2000
This gene encodes an integral membrane protein that is required for ytokineinduced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using Occludin
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.
Immunohistochemistry of paraffinn embedded human colon using
Occludin Rabbit pAb at
dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Occludin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Reviews for Occludin Rabbit Polyclonal Antibody (AB-83608)
Review for Occludin Rabbit pAb (AB-83608)