P16-INK4A Rabbit Polyclonal Antibody
| Cat Number: | AB-84778 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | cyclin-dependent kinase inhibitor 2A(melanoma, p16, inhibits CDK4) |
| Reactivity: | Human |
| Applications: | Western Blot: 1:500-1:5000 |
| Molecular Weight: | 16kDa |
| Purification: | Immunogen affinity purified |
| Synonyms: | ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, p14, p14ARF, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P19, TP16 |
| Background: | CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins have been reported, and two are related, named p16-INK4 and p14 respetively, sharing 50% identity. The third one is completely stuctually un-related, called p14(ARF) or p19(ARF). AB-84778 reacted with p16 specifically. P16 plays an important role in regulating the cell cycle, and mutations in p16 increase the risk of |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, |
| Storage: | Store at -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
HEK-293 cells were subjected to SDS PAGE followed by western blot with (P16 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using (P16-INK4A Antibody) at dilution of
1:200 heat mediated antigen retrieved with Tris- EDTA buffer(pH9).
IP Result of anti-P16 (IP:, 4ug; Detection:1:1000) with HEK-293 cells lysate 4500ug.