p21 Rabbit Polyclonal Antibody
Cat Number: | AB-83710 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160 |
Reactivity: | Human, Mouse, Rat |
Applications: | Western Blot: 1/500 – 1/2000 |
Molecular Weight: | 21kDa |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Synonyms: | CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma |
Background: | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. |
Form: | Liquid |
Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
![AB-83710-1](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-1.jpg)
Western blot analysis of lysates from COLO and K562 cells,treated with EGF, using p21 Cip1 Antibody. The lane on the right
is blocked with the synthesized peptide.
![AB-83710-2](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-2-1.jpg)
Western Blot analysis of COLO205 cells using p21 Polyclonal Antibody
![AB-83710-3](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-3.jpg)
Immunofluorescence analysis of Hela cell.
1,p21 Polyclonal Antibody(green) was diluted at 1:200(4° overnight).
2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
3 DAPI(blue) 10min.
![AB-83710-4](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-4.jpg)
Immunofluorescence analysis of mouse-kidney tissue. 1,p21 Polyclonal Antibody(red) was diluted at
1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture
B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
![AB-83710-5](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-5.jpg)
Immunofluorescence analysis of rat-lung tissue. 1,p21 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.
![AB-83710-6](https://immunologicalsciences.com/wp-content/uploads/2024/04/AB-83710-6.jpg)
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.
1,p21 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature,30min).
Negative control was used by secondary antibody only.