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Pan Acetyl Lysine Rabbit Polyclonal Antibody

Cat Number: AB-84265
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence containing acetylated K.
Reactivity: ALL SPEICES
Applications:

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Purification: Affinity purification
Background:

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved aminoterminal
domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) . Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis . Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport . The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.
Form: Liquid
Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84265-1

Immunofluorescence analysis of HeLa TSA and HeLa cells using Pan Acetyl-Lysine Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti- Rabbit IgG (H+L) at 1:500 dilution.
Blue: DAPI for nuclear staining.

References:

References for Pan Acetyl-Lysine Rabbit pAb
Product:Pan Acetyl-Lysine Rabbit pAb
Journal:Vascular Pharmacology
Application:WB
IF:5.773
Species:Mus musculus
PMID:28069546
Title:G004, a synthetic sulfonylurea compound, exerts anti-atherosclerosis effects by targeting SIRT1 in ApoE(-/-) mice

References for Pan Acetyl-Lysine Rabbit pAb
Product:Pan Acetyl-Lysine Rabbit pAb
Journal:Oxidative Medicine and Cellular Longevity
Application:WB
IF:6.543
Species:Homo sapiens
PMID:31687081
Title:Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches

References for Pan Acetyl-Lysine Rabbit pAb
Product:Pan Acetyl-Lysine Rabbit pAb
Journal:Laboratory Investigation; a Journal of Technical Methods and Pathology
Application:Co-IP
IF:5.662
Species:Mus musculus
PMID:31641228
Title:Polydatin protects against lipopolysaccharide-induced endothelial barrier disruption via SIRT3 activation

References for Pan Acetyl-Lysine Rabbit pAb
Product:Pan Acetyl-Lysine Rabbit pAb
Journal:Autophagy
Application:IP
IF:16.016
Species:Danio rerio
PMID:30205735
Title:CERKL regulates autophagy via the NAD-dependent deacetylase SIRT1

References for Pan Acetyl-Lysine Rabbit pAb
Product:Pan Acetyl-Lysine Rabbit pAb
Journal:International Journal of Molecular Medicine
Application:WB
IF:4.101
Species:Homo sapiens
PMID:31257461
Title:SIRT1 activation alleviates brain microvascular endothelial dysfunction in peroxisomal disorders.