Parvalbumin/PVALB Rabbit mAb
| Cat Number: | MAB19098 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC0385 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein.This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:1000 - 1:6000 IF-P 1:100 - 1:1000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular: | 12kDa |
| Purification: | Affinity purification |
| Synonyms: | D22S749; Parvalbumin (PVALB) |
| Background: | The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit mAb (MAB19098) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Parvalbumin (PVALB) Rabbit mAb (MAB19098) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Parvalbumin (PVALB) Rabbit mAb (MAB19098) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.