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PDHA1

Cat Number: AB-84213
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human PDHA1.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunoprecipitation: 1:20 – 1:50

Molecular: 43kDa
Purification: Aff, Pur.
Synonyms: PDHA1;PDHA;PDHAD;PDHCE1A;PHE1A
Background:

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl- CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84213-1.jpg

Western blot analysis of extracts of various cell lines, using PDHA1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.

AB-84213-2.jpg

Immunohistochemistry of paraffin embedded
human lung cancer using
PDHA1 antibody at dilution
of 1:200 (40x lens).

AB-84213-3.jpg

Immunofluorescence analysis of
NIH/3T3 cells using PDHA1 antibody
at dilution of 1:100. Blue:
DAPI for nuclear staining.