PGC1 (alpha) Rabbit Polyclonal Antibody
| Cat Number: | AB-83845 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | Polyclonal |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/100 - 1/200 - Immunofluorescence 1:50-1:100. |
| Molecular: | 102kD |
| Purification: | The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha); PGC1α |
| Background: | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide. |
| Storage: | -20°C/1 year |
Western blot analysis of various lysates, using PGC1α Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL PICO
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver using PGC1α Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
