Phospho-AKT1 (S473)

Cat Number: MAB-94003
Conjugate: Unconjugated
Size: 100 ug
Clone: D7E
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Peptide surrounding pSer-473 at the C-terminal sequence of human AKT protein
Reactivity: Hu, Ms, Rt

WB 1:1,000 ICC/IF: 1:50 IHC: 1:50

Molecular Weight: 60 kDa
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt1 protein.

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3 and (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3 -mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). Specificity/Sensitivity: Phospho-Akt1 (Ser473) (D7F10) Rabbit mAb (Akt1 Specific) recognizes endogenous levels of Akt1 protein only when phosphorylated at Ser473. It does not detect Akt2 protein when phosphorylated at Ser474.

Form: liquid
Buffer: Buffer: Supplied in 20 mM Tris HCL pH 8.0 buffer and 10 mg/mL BSA as stabilizer and 0.5% Sodium Azide. Important:. For
Storage: Store: At +4°C for short term, at-20°C for longer term Avoid freezing and thawing cycles

Representative pictures of Akt1 (only when phosphorylated at the Ser – 473 residue) in HEK293 cells, visualized with monoclonal rabbit anti -Akt1, pSer -473 monospecific antibody. Primary antibody dilution- 1:100.


Western blot analysis of extract from Hela cells untreated or treated with heat shock using Rabbit Anti-Akt – Polyclonal Antibody line 1, line 2 Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Ant.line 4, line 5