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Phospho-alpha Synuclein (S129) (15T7) Rabbit Monoclonal Antibody

Cat Number: MABN05849
Conjugate: Unconjugated
Size: 100 ug
Clone: 15T7
Concentration: 0.5mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser129 of human alpha Synuclein
Reactivity: Human,Mouse,Rat
Applications: WB 1:1000-1:5000,IHC 1:200-1:500
Molecular: 14kDa
Purification: Affinity purification
Synonyms: NACP; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; SYN; SYUA; alpha-synuclein; PARK1; PARK4; PD1; SNCA
Background:

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Form: Liquid
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage: Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.
Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate.

Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.
The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody(1/50) for 30 minutes at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody(1/50) for 30 minutes at room temperature.