|Immunogen:||A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα|
|Reactivity:||Hu, Ms, Rt, Monkey|
WB 1:1,000 IP: 1:50 IHC: 1:50
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is thecatalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellularenergy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activatedby the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of anumber of key metabolic enzymes through phosphorylation. It protects cells fromstresses that cause ATP depletion by switching off ATP-consuming biosyntheticpathways. Alternatively spliced transcript variants encoding distinct isoforms have beenobserved. [provided by RefSeq, Jul 2008]
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles|
Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat liver using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb Western blot was performed from the immunoprecipitate using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb ) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes