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Phospho-AMPKα1/2 (T183/172)

Cat Number: ABP-0116
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα
Reactivity: Hu, Ms, Rt, Monkey
Applications:

WB 1:1,000 IP: 1:50 IHC: 1:50

Molecular: 64kDa
Purification: Affinity purification
Synonyms: AMPKa1/AMPKa2
Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is thecatalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellularenergy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activatedby the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of anumber of key metabolic enzymes through phosphorylation. It protects cells fromstresses that cause ATP depletion by switching off ATP-consuming biosyntheticpathways. Alternatively spliced transcript variants encoding distinct isoforms have beenobserved. [provided by RefSeq, Jul 2008]

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
ABP-0116-1.jpg

Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat liver using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb Western blot was performed from the immunoprecipitate using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb ) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes